Different cytotoxic response to gadolinium between mouse and rat alveolar macrophages
Identifieur interne : 002467 ( Main/Exploration ); précédent : 002466; suivant : 002468Different cytotoxic response to gadolinium between mouse and rat alveolar macrophages
Auteurs : Y. Kubota [Japon] ; S. Takahashi [Japon] ; I. Takahashi [Japon] ; G. Patrick [Royaume-Uni]Source :
- Toxicology in Vitro [ 0887-2333 ] ; 2000.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
- Teeft :
- Acidic lysosomal enzyme activities, Alveolar macrophages, American journal, Apoptosis, Apoptotic cells, Balanced salt solution, Cell death, Cell lysate, Cell viability, Chloroquine, Chloroquine treatment, Colloidal form, Complete culture medium, Condensed nuclei, Contrast agent, Control culture, Corresponding concentration, Culture medium, Culture plates, Cytotoxic, Cytotoxic response, Cytotoxic sensitivity, Cytotoxicity, Data point, Dos, Dose level, Elsevier science, Environmental health perspectives, Eppendorf tube, Error bars, European journal, Gadolinium, Gadolinium chloride, Guinea pigs, Incubation temperature, Incubation time, Individual sample, International journal, Interphase cell death, Intracellular uptake, Inverted microscope, Kubota, Latex particles, Leukocyte biology, Ltered, Ltration, Lung macrophages, Lysosomotropic agents, Macrophage, Macrophage apoptosis, Many data points, Masstm ladder, Microculture plates, Molecular biology, Molecular size marker, Mouse, National institute, Nh4cl, Other hand, Particulate form, Particulate forms, Particulate matter, Phagocytic, Phagocytic activities, Phagocytic activity, Phagocytosis, Pore size, Present study, Quantitative analysis, Radiation biology, Radiological sciences, Rare earth metals, Respiratory cell, Reticuloendothelial system, Separate experiments, Standard error, Sterile saline, Subsequent dissolution, Subsequent process, Time points, Total amount, Toxicity, Viability, Viable cell number.
Abstract
Abstract: The cytotoxicity of gadolinium (Gd) chloride was investigated in alveolar macrophages (AM) cultured in vitro. A marked difference in the cytotoxic response to Gd was found between mouse and rat AM. The viability of rat AM was decreased by exposure to Gd at doses more than 3 μm, while mouse AM appeared to be resistant even up to 1000 μm Gd exposure. The decrease in the viability of rat AM exposed to Gd at doses up to 1000 μm was mitigated by centrifugation and filtration of the culture medium containing Gd, or by the treatment of AM with lysosomotropic agents such as NH4Cl or chloroquine, suggesting that the cytotoxic response of rat AM to Gd at doses up to 1000 μm was dependent on the intracellular uptake and subsequent dissolution of Gd present in the culture medium in colloidal form. The phagocytic activity of mouse AM, evaluated by the uptake of latex particles, was higher than that of rat AM. Furthermore, quantitative analysis of Gd with inductively coupled plasma-mass spectrometry revealed that mouse AM took up a larger amount of Gd than rat AM. Therefore, the marked difference in the cytotoxic response to Gd between mouse and rat AM could not be attributed to the phagocytic activities for the colloidal form of Gd. The cytotoxic sensitivity of AM to Gd present in non-colloidal form was almost the same between mouse and rat AM. Therefore, it is suggested that the extent to which Gd-colloid phagocytosed is dissolved in the phago-lysosome or the subsequent process to exhibit the cytotoxicity may be different between mouse and rat AM.
Url:
DOI: 10.1016/S0887-2333(00)00027-8
Affiliations:
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Le document en format XML
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Takahashi</name><affiliation wicri:level="3"><country xml:lang="fr">Japon</country><wicri:regionArea>Tokyo Nuclear Services Co., Ltd, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Patrick, G" sort="Patrick, G" uniqKey="Patrick G" first="G" last="Patrick">G. Patrick</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>MRC Toxicology Unit, Leicester</wicri:regionArea><wicri:noRegion>Leicester</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Toxicology in Vitro</title><title level="j" type="abbrev">TIV</title><idno type="ISSN">0887-2333</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000">2000</date><biblScope unit="volume">14</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="309">309</biblScope><biblScope unit="page" to="319">319</biblScope></imprint><idno type="ISSN">0887-2333</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0887-2333</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animal</term><term>Apoptosis</term><term>Cell death</term><term>Colloidal state</term><term>Cytotoxicity</term><term>Gadolinium</term><term>In vitro</term><term>Interspecific comparison</term><term>Lung</term><term>Macrophage</term><term>Mouse</term><term>Phagocytosis</term><term>Rat</term><term>Toxicity</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Animal</term><term>Apoptose</term><term>Comparaison interspécifique</term><term>Cytotoxicité</term><term>Etat colloïdal</term><term>Gadolinium</term><term>In vitro</term><term>Macrophage</term><term>Mort cellulaire</term><term>Phagocytose</term><term>Poumon</term><term>Rat</term><term>Souris</term><term>Toxicité</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Acidic lysosomal enzyme activities</term><term>Alveolar macrophages</term><term>American journal</term><term>Apoptosis</term><term>Apoptotic cells</term><term>Balanced salt solution</term><term>Cell death</term><term>Cell lysate</term><term>Cell viability</term><term>Chloroquine</term><term>Chloroquine treatment</term><term>Colloidal form</term><term>Complete culture medium</term><term>Condensed nuclei</term><term>Contrast agent</term><term>Control culture</term><term>Corresponding concentration</term><term>Culture medium</term><term>Culture plates</term><term>Cytotoxic</term><term>Cytotoxic response</term><term>Cytotoxic sensitivity</term><term>Cytotoxicity</term><term>Data point</term><term>Dos</term><term>Dose level</term><term>Elsevier science</term><term>Environmental health perspectives</term><term>Eppendorf tube</term><term>Error bars</term><term>European journal</term><term>Gadolinium</term><term>Gadolinium chloride</term><term>Guinea pigs</term><term>Incubation temperature</term><term>Incubation time</term><term>Individual sample</term><term>International journal</term><term>Interphase cell death</term><term>Intracellular uptake</term><term>Inverted microscope</term><term>Kubota</term><term>Latex particles</term><term>Leukocyte biology</term><term>Ltered</term><term>Ltration</term><term>Lung macrophages</term><term>Lysosomotropic agents</term><term>Macrophage</term><term>Macrophage apoptosis</term><term>Many data points</term><term>Masstm ladder</term><term>Microculture plates</term><term>Molecular biology</term><term>Molecular size marker</term><term>Mouse</term><term>National institute</term><term>Nh4cl</term><term>Other hand</term><term>Particulate form</term><term>Particulate forms</term><term>Particulate matter</term><term>Phagocytic</term><term>Phagocytic activities</term><term>Phagocytic activity</term><term>Phagocytosis</term><term>Pore size</term><term>Present study</term><term>Quantitative analysis</term><term>Radiation biology</term><term>Radiological sciences</term><term>Rare earth metals</term><term>Respiratory cell</term><term>Reticuloendothelial system</term><term>Separate experiments</term><term>Standard error</term><term>Sterile saline</term><term>Subsequent dissolution</term><term>Subsequent process</term><term>Time points</term><term>Total amount</term><term>Toxicity</term><term>Viability</term><term>Viable cell number</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The cytotoxicity of gadolinium (Gd) chloride was investigated in alveolar macrophages (AM) cultured in vitro. A marked difference in the cytotoxic response to Gd was found between mouse and rat AM. The viability of rat AM was decreased by exposure to Gd at doses more than 3 μm, while mouse AM appeared to be resistant even up to 1000 μm Gd exposure. The decrease in the viability of rat AM exposed to Gd at doses up to 1000 μm was mitigated by centrifugation and filtration of the culture medium containing Gd, or by the treatment of AM with lysosomotropic agents such as NH4Cl or chloroquine, suggesting that the cytotoxic response of rat AM to Gd at doses up to 1000 μm was dependent on the intracellular uptake and subsequent dissolution of Gd present in the culture medium in colloidal form. The phagocytic activity of mouse AM, evaluated by the uptake of latex particles, was higher than that of rat AM. Furthermore, quantitative analysis of Gd with inductively coupled plasma-mass spectrometry revealed that mouse AM took up a larger amount of Gd than rat AM. Therefore, the marked difference in the cytotoxic response to Gd between mouse and rat AM could not be attributed to the phagocytic activities for the colloidal form of Gd. The cytotoxic sensitivity of AM to Gd present in non-colloidal form was almost the same between mouse and rat AM. Therefore, it is suggested that the extent to which Gd-colloid phagocytosed is dissolved in the phago-lysosome or the subsequent process to exhibit the cytotoxicity may be different between mouse and rat AM.</div></front></TEI><affiliations><list><country><li>Japon</li><li>Royaume-Uni</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><country name="Japon"><noRegion><name sortKey="Kubota, Y" sort="Kubota, Y" uniqKey="Kubota Y" first="Y" last="Kubota">Y. Kubota</name></noRegion><name sortKey="Kubota, Y" sort="Kubota, Y" uniqKey="Kubota Y" first="Y" last="Kubota">Y. Kubota</name><name sortKey="Takahashi, I" sort="Takahashi, I" uniqKey="Takahashi I" first="I" last="Takahashi">I. Takahashi</name><name sortKey="Takahashi, S" sort="Takahashi, S" uniqKey="Takahashi S" first="S" last="Takahashi">S. Takahashi</name></country><country name="Royaume-Uni"><noRegion><name sortKey="Patrick, G" sort="Patrick, G" uniqKey="Patrick G" first="G" last="Patrick">G. Patrick</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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